Effects of Recombinant Human AMH on SCF Expression in Human Granulosa Cells

The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of S...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2013-12, Vol.67 (3), p.1481-1485
Main Authors: Hu, Rong, Lou, Yan, Wang, Fei-Miao, Ma, Hui-Ming, Wu, Xing, Zhang, Xiao-Mei, Li, Juan, Sun, Ying-Pu
Format: Article
Language:English
Subjects:
Adult
Anti-Mullerian Hormone - genetics
Anti-Mullerian Hormone - metabolism
Anti-Mullerian Hormone - pharmacology
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Cell Biology
Cells, Cultured
Female
Granulosa Cells - cytology
Granulosa Cells - drug effects
Granulosa Cells - metabolism
Humans
Life Sciences
Pharmacology/Toxicology
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - pharmacology
RNA, Messenger - metabolism
Stem Cell Factor - genetics
Stem Cell Factor - metabolism
Stem cells
Translational Biomedical Research
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Summary:The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group ( p  
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-013-9649-x