Purification and Partial Characterization of Bacillomycin L Produced by Bacillus amyloliquefaciens K103 from Lemon

• Published in: Applied biochemistry and biotechnology 2013-12, Vol.171 (8), p.2262-2272
• Main Authors: Zhang, Bao, Dong, Chunjuan, Shang, Qingmao, Cong, Yuan, Kong, Weijia, Li, Pinglan
• Format: Article
• Language: English
• Subjects: acid deposition; Agricultural biotechnology; amino acid composition; Amino Acids; Antibiotics; antifungal agents; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Bacillus - chemistry; Bacillus - metabolism; Bacillus amyloliquefaciens; Biochemistry; Biological and medical sciences; Biological control; biological control agents; Biotechnology; Chemistry; Chemistry and Materials Science; Citrus - chemistry; Citrus - microbiology; desorption; Food science; foods; Fundamental and applied biological sciences. Psychology; gel permeation chromatography; Ionization; isomers; lemons; Liquid chromatography; Mass spectrometry; Molecular Weight; Peptides, Cyclic - chemistry; Peptides, Cyclic - isolation & purification; plant pathogens; reversed-phase high performance liquid chromatography; Rhizoctonia - drug effects; Sustainable agriculture; Thanatephorus cucumeris
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-013-0424-7

Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance liquid chromatography. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis indicated that the antifungal compounds were two isomers similar to bacillomycin L. One of the predominant active fractions was subjected to quadrupole time-of-flight mass spectrometry and amino acid analysis to determine its structural characteristics, revealing that the antifungal compound with a molecular mass of 1,034.5464 was identical to bacillomycin L. This is the second report of lemon microflora producing bacillomycin L or any antifungal compound, suppressing the growth of R. solani Kühn. Meanwhile, the study provided insights into the enormous potential of food microbial resources and bacillomycin L antibiotics in biological control and sustainable agriculture.