Enhancement of 4‑Acetylantroquinonol B Production by Supplementation of Its Precursor during Submerged Fermentation of Antrodia cinnamomea

The antiproliferation activity of the ethanol extract of A. cinnamomea mycelium on hepatocellular cancer cells HepG2 was found to be associated with aroma intensity of the broth during fermentation. We hypothesized that some of the volatile compounds are the precursors of the key bioactive component...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2013-09, Vol.61 (38), p.9160-9165
Main Authors: Chiang, Chien-Chi, Huang, Tzu-Ning, Lin, Yu-Wei, Chen, Kai-Hsien, Chiang, Been-Huang
Format: Article
Language:English
Subjects:
4-Butyrolactone - analogs & derivatives
4-Butyrolactone - analysis
4-Butyrolactone - biosynthesis
4-Butyrolactone - pharmacology
Antineoplastic Agents - analysis
Antineoplastic Agents - metabolism
Antrodia - chemistry
Antrodia - metabolism
bioactive properties
Biological and medical sciences
Cell Proliferation - drug effects
Culture Media - chemistry
Culture Media - metabolism
Cyclohexanones - analysis
Cyclohexanones - pharmacology
ethanol
Fermentation
Food industries
Fundamental and applied biological sciences. Psychology
fungi
Gas Chromatography-Mass Spectrometry
Hep G2 Cells
hepatoma
Humans
linalool
mycelium
odors
submerged fermentation
Taiwanofungus camphoratus
volatile compounds
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Summary:The antiproliferation activity of the ethanol extract of A. cinnamomea mycelium on hepatocellular cancer cells HepG2 was found to be associated with aroma intensity of the broth during fermentation. We hypothesized that some of the volatile compounds are the precursors of the key bioactive component 4-acetylantroquinonol B of this fungus. The major volatile compounds of A. cinnamomea were identified by GC/MS, and they are oct-1-en-3-ol, linalool, methyl phenylacetate, nerolidol, γ-cadinene and 2,4,5-trimethoxybenzaldehyde (TMBA). TMBA and nerolidol were further selected and used as supplements during fermentation. It was found that both of them could increase the production of 4-acetylantroquinonol B and enhance the antiproliferation activity of the fungus. In addition, the TMBA was identified as the most promising supplement for increasing the bioactivity of A. cinnamomea during cultivation.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf402187q