Recapitulation of characteristics of human placental vascular insufficiency in a novel mouse model

Abstract Objective We tested the effects of selective reduction of placental blood flow by mesenteric uterine artery branch ligation (MUAL) resulting in fetal growth restriction (FGR). Methods Timed mated C57BL/6J Day( D ) 18 dams were divided into two groups: MUAL ( n  = 18); and control-sham ( n  ...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2013-12, Vol.34 (12), p.1150-1158
Main Authors: Habli, M, Jones, H, Aronow, B, Omar, K, Crombleholme, T.M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth Weight
Disease Models, Animal
Embryology: invertebrates and vertebrates. Teratology
Female
Fetal growth restriction
Fetal Growth Retardation - etiology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
IGF
Insulin-Like Growth Factor Binding Proteins - genetics
Insulin-Like Growth Factor Binding Proteins - metabolism
Internal Medicine
Ligation
Male
Mice
Mice animal model
Mice, Inbred C57BL
Obstetrics and Gynecology
Placenta - metabolism
Placenta - pathology
Placenta - physiopathology
Placental Circulation
Placental insufficiency
Placental Insufficiency - metabolism
Placental Insufficiency - pathology
Placental Insufficiency - physiopathology
Placentation
Pregnancy
Sex Characteristics
Somatomedins - genetics
Somatomedins - metabolism
Uterine Artery - surgery
Weight Gain
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Summary:Abstract Objective We tested the effects of selective reduction of placental blood flow by mesenteric uterine artery branch ligation (MUAL) resulting in fetal growth restriction (FGR). Methods Timed mated C57BL/6J Day( D ) 18 dams were divided into two groups: MUAL ( n  = 18); and control-sham ( n  = 18). Pups were delivered on D20, cross-fostered to surrogate CD-1 mothers for 4 weeks, and followed for 8 weeks. Outcome data included birth and placental weight, postnatal growth, placental volume determined by stereology, quantification of placental insulin-like growth factors-1(IGF-1) and IGF-2 and IGF binding proteins(IGFBP 2 and 6) by ELISA and gene expression by qPCR and GeneChip microarray analysis. Results Compared with control, MUAL had an 11% reduction in mean birth weight (1.06 ± 0.13 g vs. 0.94 ± 0.13 g, p  
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2013.09.011