Fabrication of high-density collagen fibril matrix gels by renaturation of triple-helix collagen from gelatin

Collagen-based 3-D hydrogels often lack sufficient mechanical strength for tissue engineering. We developed a method for fabrication of high-density collagen fibril matrix (CFM) gels from concentrated solutions of uncleaved gelatin (UCG). Denatured random-coil UCG exhibited more rapid and efficient...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2013-11, Vol.62, p.296-303
Main Authors: Ohyabu, Yoshimi, Yunoki, Shunji, Hatayama, Hirosuke, Teranishi, Yoshikazu
Format: Article
Language:English
Subjects:
Animals
Cell encapsulation
Cell Line
Cell Survival - drug effects
Collagen
Collagen - chemistry
Collagen - pharmacology
Fibril
Gelatin
Gelatin - chemistry
Gels
Hot Temperature
Humans
Protein Renaturation
Protein Structure, Secondary
Renaturation
Temperature
Three-dimensional cell culture
Tissue Engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Collagen-based 3-D hydrogels often lack sufficient mechanical strength for tissue engineering. We developed a method for fabrication of high-density collagen fibril matrix (CFM) gels from concentrated solutions of uncleaved gelatin (UCG). Denatured random-coil UCG exhibited more rapid and efficient renaturation into collagen triple-helix than cleaved gelatin (CG) over a broad range of setting temperatures. The UCG solution formed opaque gels with high-density reconstituted collagen fibrils at 28–32°C and transparent gels similar to CG at 5%) and elasticity (1.28±0.15kPa at 5% and 4.82±0.38kPa at 8%) with minimal cell loss. The elastic modulus of these gels was higher than that of conventional CFM containing 0.5% collagen. High-strength CFM may provide more durable hydrogels for tissue engineering and regenerative medicine.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2013.09.001