Chemical analysis and antioxidant activity of polysaccharides extracted from Inonotus obliquus sclerotia

Three water-soluble polysaccharide fractions (IOP40, IOP60 and IOP80) were isolated by using different concentrations of alcohol precipitation from Inonotus obliquus sclerotia. Their physicochemical properties, including total sugar content, protein content, monosaccharide composition and percentage...

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Bibliographic Details
Published in:International journal of biological macromolecules 2013-11, Vol.62, p.691-696
Main Authors: Du, XiuJu, Mu, HongMei, Zhou, Shuai, Zhang, Yang, Zhu, XiaoLi
Format: Article
Language:English
Subjects:
2,2-diphenyl-1-picrylhydrazyl
Antioxidant activity
Antioxidants - chemistry
Antioxidants - pharmacology
Basidiomycota - chemistry
chemical analysis
Free Radical Scavengers - chemistry
Free radicals scavenging activity
functional foods
Fungal Polysaccharides - chemistry
Fungal Polysaccharides - pharmacology
hydrogen peroxide
Hydrogen Peroxide - antagonists & inhibitors
Hydroxyl Radical - antagonists & inhibitors
hydroxyl radicals
Inonotus obliquus
Inonotus obliquus polysaccharide
Liquid-Liquid Extraction - methods
Monosaccharide composition
oxidative stress
pharmaceutical industry
polysaccharides
protein content
sclerotia
sugar content
superoxide anion
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Summary:Three water-soluble polysaccharide fractions (IOP40, IOP60 and IOP80) were isolated by using different concentrations of alcohol precipitation from Inonotus obliquus sclerotia. Their physicochemical properties, including total sugar content, protein content, monosaccharide composition and percentage were analyzed. And their in vitro antioxidant capacities were investigated in terms of reducing power assay and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, hydroxyl radicals, superoxide anion radicals and hydrogen peroxide (H2O2). In general, three polysaccharide fractions exhibited increasing antioxidant activity with increasing concentration at the ranges of tested dosage. The orders of reducing power, DPPH-scavenging capacity, H2O2-scavenging capacity, and hydroxyl-scavenging activity were all IOP60>IOP40>IOP80. These findings demonstrated that three polysaccharide fractions extracted from I. obliquus, especially IOP60, could be employed as natural ingredients in functional food and pharmaceutical industry to alleviate the oxidative stress.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2013.10.016