Effects of Bacillus subtilis metabolites on larval Aedes aegypti L

•An extracellular secondary metabolites was purified from Bacillus subtilis a gram-positive isolates from the soil samples.•The secondary metabolites were purified and molecular masses were determined through SDS–PAGE, 16S rRNA and HPLC.•The lethal concentrations were identified for bioassay against...

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Published in:Pesticide biochemistry and physiology 2013-11, Vol.107 (3), p.369-376
Main Authors: Revathi, Kannan, Chandrasekaran, Rajamanickam, Thanigaivel, Annamalai, Kirubakaran, Suyambulingam Arunachalam, Sathish-Narayanan, Subbiah, Senthil-Nathan, Sengottayan
Format: Article
Language:English
Subjects:
16S rRNA
Acetylcholinesterase - metabolism
Acid Phosphatase - metabolism
Aedes - drug effects
Aedes aegypti
Animals
Bacillus subtilis
Bacillus subtilis - metabolism
Digestive enzyme
Enzyme Activation - drug effects
Insect Proteins - metabolism
Insecticides - metabolism
Insecticides - pharmacology
Metabolites
Mortality
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Summary:•An extracellular secondary metabolites was purified from Bacillus subtilis a gram-positive isolates from the soil samples.•The secondary metabolites were purified and molecular masses were determined through SDS–PAGE, 16S rRNA and HPLC.•The lethal concentrations were identified for bioassay against the dengue vector Aedes aegypti.•Effect of B. subtilis secondary metabolites was determined through AChE, α and β carboxylesterase, ACP and ALP. The culture supernatant of a strain of Bacillus subtilis isolated from soil samples killed larvae of the mosquito Aedes aegypti. The metabolites produced by B. subtilis were characterized using high performance liquid chromatography (HPLC). Mortality rate was dose-dependent for all larval instars of A. aegypti. Log probit analysis (95% confidence level) revealed an LC50 of 1.73 and an LC90 3.71μg/ml. Molecular weights/masses of B. subtilis metabolites were confirmed using SDS–PAGE analysis. B. subtilis metabolites were confirmed using HPLC analysis. We demonstrate that secondary metabolites from B. subtilis have larvicidal activity against A. aegypti and may be suitable for the control of this and other mosquito vectors of human disease. The larvae to the metabolites, significant reduction in the activities of acetylcholinesterse, α-carboxylesterase, and acid phosphatases were recorded.
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2013.10.005