Chitosan–hyaluronic acid/nano silver composite sponges for drug resistant bacteria infected diabetic wounds

• Published in: International journal of biological macromolecules 2013-11, Vol.62, p.310-320
• Main Authors: Anisha, B.S., Biswas, Raja, Chennazhi, K.P., Jayakumar, R.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Bacteria - cytology; Bacteria - drug effects; Bandages - microbiology; Cell Adhesion - drug effects; Cell Survival - drug effects; Chitosan - chemistry; Chitosan–hyaluronic acid; Diabetic Foot - microbiology; Drug Resistance, Bacterial - drug effects; Fibroblasts - cytology; Fibroblasts - drug effects; Humans; Hyaluronic Acid - chemistry; Membrane Potentials - drug effects; Metal Nanoparticles - chemistry; Nano silver; Nanocomposites - chemistry; Porosity; Silver - chemistry; Water - chemistry; Wound dressing-sponge-diabetic foot ulcers; Wound Healing - drug effects
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2013.09.011

The aim of this work was to develop an antimicrobial sponge composed of chitosan, hyaluronic acid (HA) and nano silver (nAg) as a wound dressing for diabetic foot ulcers (DFU) infected with drug resistant bacteria. nAg (5–20nm) was prepared and characterized. The nanocomposite sponges were prepared by homogenous mixing of chitosan, HA and nAg followed by freeze drying to obtain a flexible and porous structure. The prepared sponges were characterized using SEM and FT-IR. The porosity, swelling, biodegradation and haemostatic potential of the sponges were also studied. Antibacterial activity of the prepared sponges was analysed using Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Klebsiella pneumonia. Chitosan–HA/nAg composite sponges showed potent antimicrobial property against the tested organisms. Sponges containing higher nAg (0.005%, 0.01% and 0.02%) concentrations showed antibacterial activity against MRSA. Cytotoxicity and cell attachment studies were done using human dermal fibroblast cells. The nanocomposite sponges showed a nAg concentration dependent toxicity towards fibroblast cells. Our results suggest that this nanocomposite sponges could be used as a potential material for wound dressing for DFU infected with antibiotic resistant bacteria if the optimal concentration of nAg exhibiting antibacterial action with least toxicity towards mammalian cells is identified.