Regulation of swelling of etiolated wheat-leaf protoplasts by phytochrome and gibberellic acid

The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was ph...

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Bibliographic Details
Published in:Planta 1983-08, Vol.158 (5), p.416-421
Main Authors: Blakeley, S D, Thomas, B, Hall, J L, Vince-Prue, D
Format: Article
Language:English
Subjects:
Cell membranes
Cell walls
Chromium
Gibberellins
Plant cells
Plants
Protoplasts
Solutes
Swelling
Viability
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Summary:The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4° C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m-2 s-1 was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00397734