Cell wall localization of dihydroflavonol-glucoside beta-glucosidase in flowers of Petunia hybrida

Limbs of flower buds from Petunia hybrida were investigated for β-glucosidase activity with dihydroflavonol-glucosides and 4-methyl-umbelliferylβ-D-glucoside as substrates. Dihydroflavonol-glucoside β-glucosidase is localized in the cell wall. This activity has an acid pH optimum and is also active...

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Bibliographic Details
Published in:Planta 1982-07, Vol.155 (2), p.162-165
Main Authors: Schram, A.W, Al, E.J.M, Douma, N, Jonsson, L.M.V, Vlaming, P. de, Kooi, A, Bennink, G.J.H
Format: Article
Language:English
Subjects:
Cell walls
Electrophoresis
Enzyme activity
Enzyme substrates
Enzymes
Flavonoids
Flowering
Gels
Glucosides
plant biochemistry
plant physiology
Starches
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Summary:Limbs of flower buds from Petunia hybrida were investigated for β-glucosidase activity with dihydroflavonol-glucosides and 4-methyl-umbelliferylβ-D-glucoside as substrates. Dihydroflavonol-glucoside β-glucosidase is localized in the cell wall. This activity has an acid pH optimum and is also active toward 4-methyl-umbelliferyl-β-glucoside. Besides this activity a neutral β-glucosidase is present. This activity is soluble and is not active toward dihydroflavonol-glucosides. Using starch gel electrophoresis it was shown that no difference in β-glucosidase activity is present between mutants able to convert dihydroflavonols into anthocyanins and mutants accumulating dihydroflavonol-glucosides. It is concluded that β-glucosidase activity is not involved in anthocyanin synthesis.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00392547