Influence of growth conditions on the composition of cell wall polysaccharides from cultured tobacco cells

The sugar composition of cell wall polysaccharides of two tobacco varieties obtained from mesophyll, regenerating protoplasts and cells grown under various conditions were compared. Regenerating protoplasts developed an unusual cell wall with a low cellulose and a high non-cellulosic glucan content....

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Bibliographic Details
Published in:Plant cell reports 1983-10, Vol.2 (5), p.257-260
Main Authors: BLASCHEK, W, FRANZ, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cell cultures. Hybridization. Fusion
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
In vitro propagation: entire plant regeneration from tissues and cell cultures
Methods. Procedures. Technologies
Molecular and cellular biology
Plant cells and fungal cells
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Summary:The sugar composition of cell wall polysaccharides of two tobacco varieties obtained from mesophyll, regenerating protoplasts and cells grown under various conditions were compared. Regenerating protoplasts developed an unusual cell wall with a low cellulose and a high non-cellulosic glucan content. In the presence of different phytohormones compact and friable calli were obtained with cell walls containing low and high arabinose/xylose ratios. The cell walls of compact calli were comparable to those of genuine mesophyll cells. The sugar constituents of cell walls obtained from cells grown in liquid media were different from those of solid calli. The cell wall composition of suspension cultured cells was hardly affected by various combinations of phytohormones, but was altered by high osmolarity of the medium.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00269155