Pectic polysaccharides in carrot cells growing in suspension culture

Pectic polysaccharides in the cell wall of suspension-cultured carrot cells (Daucus carota L.) were fractionated into high- and low-molecular-weight components by molecular-sieve chromatography with a Sepharose 4B column. During the phase of cell-wall expansion, the relative content of low-molecular...

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Bibliographic Details
Published in:Planta 1984-04, Vol.160 (5), p.469-473
Main Authors: Asamizu, Tetsuya, Nakayama, Noriko, Nishi, Arasuke
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Cell culture techniques
Cell growth
Cell wall components
Cell walls
Cultured cells
Economic plant physiology
Fundamental and applied biological sciences. Psychology
In vitro culture
Plant cells
Plant physiology and development
Polymers
Polysaccharides
Sugars
Tissue cultures, protoplasts
Uronic acids
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Summary:Pectic polysaccharides in the cell wall of suspension-cultured carrot cells (Daucus carota L.) were fractionated into high- and low-molecular-weight components by molecular-sieve chromatography with a Sepharose 4B column. During the phase of cell-wall expansion, the relative content of low-molecular-weight polymers rapidly increased. Electrophoretic analyses of these fractions showed that the high-molecular-weight components were largely composed of neutral and weakly acidic polymers while the low-molecular-weight fraction contained, in addition to neutral polymers, strongly acidic polyuronides in which the content of neutral sugars was very small. The accumulation of a large amount of the strongly acidic polyuronides occurred in a late stage of cell-wall growth, concomitant with a marked decrease in the high-molecular-weight components.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00429765