Chitosan–guar gum–silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system

• Published in: Journal of pharmaceutical and biomedical analysis 2014-01, Vol.88, p.571-578
• Main Authors: Bagal-Kestwal, Dipali R., Kestwal, Rakesh Mohan, Hsieh, Wen-Ting, Chiang, Been-Huang
• Format: Article
• Language: English
• Subjects: Basidiomycota - metabolism; beta-Glucans - chemistry; Biopolymers - chemistry; Bioreactors; Calibration; Chitosan - chemistry; Chitosan–guar gum composite beads; Cost-Benefit Analysis; Enzymes, Immobilized - chemistry; Fabaceae - enzymology; Fermentation; Flow injection analysis; Galactans - chemistry; Glucans - chemistry; Glucose - chemistry; Glucose Oxidase - chemistry; Hydrogen-Ion Concentration; Mannans - chemistry; Metal Nanoparticles - chemistry; Microscopy, Electron, Transmission; Nanocomposites - chemistry; Peroxidase - chemistry; Photometry; Plant Gums - chemistry; Reproducibility of Results; Silver - chemistry; Silver nanoparticles; Spectrophotometry; β-(1 → 3)-d-glucan detection; β-(1 → 3)-d-glucanase
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2013.09.011

•β-(1→3)-d-Glucan from Ganoderma lucidum is an immunomodulator.•Purified plant glucanase–glucose oxidase immobilized in novel biopolymer composite.•Spectrophotometric FIA using silver nanoparticles–chitosan–guar gum enzyme bead reactors.•FIA sensing system validated for dual estimation of β-glucan and glucose.•Designed system used for β-glucan estimation in G. lucidum fermentation broth. Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation. For this purpose, the chitosan–guar gum–silver nanoparticle–beta glucanase (Ch–GG–AgNPs–βG) beads and Ch–GG–AgNPs–GOD (glucose oxidase) beads were prepared. The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose. This colorimetric flow system can detect glucose and glucan with detection limits as low as 50ngmL−1 and 100ngmL−1 (S/N=3), respectively. The analysis time of this FIA was approximately 40s, which is faster than the previously reported glucan sensors. The glucose and glucan calibration curves were obtained in the range of 0.25–1.25μgmL−1 (R2=0.988) and 0.2–1.0μgmL−1(R2=0.979), respectively. The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%. Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.