Validation of a method for the targeted analysis of 96 drugs in hair by UPLC–MS/MS

•The method allows the targeted screening and quantification of 96 drugs in hair.•Possible detection of single-dose drug exposure for some analytes.•Comprehensive method due to simple sample preparation and wide linear range. The method presented in this study allows the screening and quantification...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2014-01, Vol.88, p.295-306
Main Authors: Montesano, Camilla, Johansen, Sys Stybe, Nielsen, Marie Katrine Klose
Format: Article
Language:English
Subjects:
Acetonitriles - chemistry
Calibration
Chromatography, High Pressure Liquid
Drugs of abuse
Formates - chemistry
Hair
Hair - chemistry
Humans
Ions
Linear Models
Methanol - chemistry
Pharmaceutical Preparations - analysis
Pharmaceuticals
Quality Control
Reproducibility of Results
Solvents
Street Drugs - analysis
Substance Abuse Detection - methods
Tandem Mass Spectrometry
Targeted screening and quantification
UPLC–MS/MS
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Summary:•The method allows the targeted screening and quantification of 96 drugs in hair.•Possible detection of single-dose drug exposure for some analytes.•Comprehensive method due to simple sample preparation and wide linear range. The method presented in this study allows the screening and quantification of 96 drugs, from different groups: opiates, amphetamines, hallucinogens, benzodiazepines, antihistamines, antidepressants, antipsychotics, barbiturates and other sedatives, muscle relaxants, etc. in hair. Drugs are extracted from 10mg of washed hair in 18h by a mixture of methanol:acetonitrile:ammonium formate (pH 5.3). Absolute recovery ranged from 70% to 106% for 75% of the analytes. The limits of detection in the low pg/mg range, may allow the detection of single dose drug exposure, with possible application in drug facilitated assaults (DFA); however, chronic use (compliance) can also be examined. The method has been fully validated for the drugs included in the study. The accuracy of the method was demonstrated by the analysis of certified authentic hair samples containing common drugs of abuse. The hair-method has broad potential as the measuring range is wide for the target analytes and new drugs can easily be added to the method due to the versatility of the extraction procedure and chromatographic system.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2013.08.050