Purification, antioxidant activity and antiglycation of polysaccharides from Polygonum multiflorum Thunb

• Published in: Carbohydrate polymers 2014, Vol.99, p.765-773
• Main Authors: Lv, Lishuang, Cheng, Yunhui, Zheng, Tiesong, Li, Xiaoming, Zhai, Rong
• Format: Article
• Language: English
• Subjects: Animals; Antiglycation; Antioxidant; Applied sciences; Biological and medical sciences; Chromatography, Gel; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Exact sciences and technology; Free Radical Scavengers - chemistry; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - pharmacology; General and cellular metabolism. Vitamins; Glycation End Products, Advanced - antagonists & inhibitors; Glycation End Products, Advanced - metabolism; Glycosylation - drug effects; Heart - drug effects; Hydroxyl Radical - antagonists & inhibitors; Kidney - drug effects; Kidney - metabolism; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - metabolism; Medical sciences; Molecular Weight; Natural polymers; Oxidation-Reduction; Pharmacology. Drug treatments; Physicochemistry of polymers; Polygonum - chemistry; Polygonum multiflorum Thunb; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Rats; Rats, Sprague-Dawley; Starch and polysaccharides; Superoxides - antagonists & inhibitors
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.09.007

•Two polysaccharides were purified from Polygonum multiflorum Thunb.•The polysaccharides can scavenge free radicals and inhibit lipid oxidation.•The polysaccharides can inhibit protein glycation. Polysaccharides, one of the most important constituents in Polygonum multiflorum Thunb, a famous Chinese medicinal herb, were isolated by DEAE-52, Sepharose 4B and Sephacryl S-300 column chromatography. Two polysaccharides (PMP-1 and PMP-2) were identified as homogeneous in molecular weight with HPLC. The molecular weights were 4.8×102 and 6.1×102kDa, respectively. Antioxidant activity tests were performed with two polysaccharides at concentrations of 0.1–1.5mg/mL. The results indicated that the inhibitory activity on oxidation and glycation exhibited a dose-dependent response. PMP-2 exhibited a much stronger antioxidant capacity against free radical, lipid oxidation and protein glycation. The IC50 values of PMP-2 were 0.47, 0.6 and 0.93mg/mL for superoxide anion scavenging, hydroxyl radical scavenging, and hydroxyl peroxide scavenging, respectively. The inhibitory ability of PMP-2 on lipid oxidation was most markedly in rat liver, followed by heart and kidney. Meanwhile, PMP-2 also showed satisfactory suppression of AGEs formation. This suggested that the polysaccharides present in PM can contribute to the biological effects.