Assessing leaf photoprotective mechanisms using terrestrial LiDAR: towards mapping canopy photosynthetic performance in three dimensions

• Published in: The New phytologist 2014-01, Vol.201 (1), p.344-356
• Main Authors: Magney, Troy S., Eusden, Spencer A., Eitel, Jan U. H., Logan, Barry A., Jiang, Jingjue, Vierling, Lee A.
• Format: Article
• Language: English
• Subjects: Canopies; Canopy; Chlorophyll; Chlorophyll - metabolism; Chlorophylls; Dimensions; Epoxidation; Fluorescence; Forest canopy; green laser return intensity (GLRI); Herbivores; Laser applications; Lasers; Leaf angle; leaf pigments; Leaves; Lidar; Light; light detection and ranging (LiDAR); Light intensity; Luminous intensity; Methods; Narrowband; non‐photochemical quenching (NPQ); photochemical reflectance index (PRI); Photochemicals; Photochemistry; Photosynthesis; Photosynthetic Reaction Center Complex Proteins - metabolism; Physiology; Plant Leaves - metabolism; Plant Leaves - physiology; Plant Physiological Phenomena; Plant species; Plants; Reflectance; Remote sensing; Scaling; Spectral reflectance; Terrestrial environments; terrestrial laser scanning (TLS); Vegetation canopies; Violaxanthin; xanthophyll cycle; Xanthophylls; Xanthophylls - metabolism; Zeaxanthin
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.12453

Terrestrial laser scanning (TLS) data allow spatially explicit (x, y, z) laser return intensities to be recorded throughout a plant canopy, which could considerably improve our understanding of how physiological processes vary in three-dimensional space. However, the utility of TLS data for the quantification of plant physiological properties remains largely unexplored. Here, we test whether the laser return intensity of green (532-nm) TLS correlates with changes in the de-epoxidation state of the xanthophyll cycle and photoprotective non-photochemical quenching (NPQ), and compare the ability of TLS to quantify these parameters with the passively measured photochemical reflectance index (PRI). We exposed leaves from five plant species to increasing light intensities to induce NPQ and de-epoxidation of violaxanthin (V) to antheraxanthin (A) and zeaxanthin (Z). At each light intensity, the green laser return intensity (GLRI), narrowband spectral reflectance, chlorophyll fluorescence emission and xanthophyll cycle pigment composition were recorded. Strong relationships between both predictor variables (GLRI, PRI) and both explanatory variables (NPQ, xanthophyll cycle de-epoxidation) were observed. GLRI holds promise to provide detailed (mm) information about plant physiological status to improve our understanding of the patterns and mechanisms driving foliar photoprotection. We discuss the potential for scaling these laboratory data to three-dimensional canopy space.