Determination of pK(a) values of a triptolide derivative and its impurities by pressure-assisted capillary electrophoresis

A pressure-assisted capillary zone electrophoresis method was developed to determine the pKa values of a triptolide derivative (LLDT-246) and its impurities. The method was performed in an uncoated fused-silica capillary under the electric voltage of 18kV and 50mbar of external pressure applied simu...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2014-01, Vol.88, p.22-26
Main Authors: Wang, Jian-Ling, Xu, Xue-Jiao, Chen, Dong-Ying
Format: Article
Language:English
Subjects:
Automation
Buffers
Chemistry, Pharmaceutical
Diterpenes - analysis
Diterpenes - chemistry
Drug Design
Electrochemistry
Electrolytes
Electrophoresis, Capillary - methods
Epoxy Compounds - analysis
Epoxy Compounds - chemistry
Hydrogen-Ion Concentration
Immunosuppressive Agents - analysis
Ions
Models, Chemical
Osmolar Concentration
Phenanthrenes - analysis
Phenanthrenes - chemistry
Plant Extracts - analysis
Pressure
Tripterygium - metabolism
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Summary:A pressure-assisted capillary zone electrophoresis method was developed to determine the pKa values of a triptolide derivative (LLDT-246) and its impurities. The method was performed in an uncoated fused-silica capillary under the electric voltage of 18kV and 50mbar of external pressure applied simultaneously. A series of running electrolyte buffers were used with pH ranging between 2.2 and 10.0 with the constant ionic strength of 0.05M. The values of pKa of LLDT-246 and two impurities were calculated based on the pH dependence of effective mobilities (μeff). The pKa value of LLDT-246 was in good agreement with that of determined by potentiometric titration method.
ISSN:1873-264X
DOI:10.1016/j.jpba.2013.08.012