The expression and prognosis of Emi1 and Skp2 in breast carcinoma: associated with PI3K/Akt pathway and cell proliferation

• Published in: Medical oncology (Northwood, London, England) London, England), 2013-12, Vol.30 (4), p.735-735, Article 735
• Main Authors: Liu, Xiaobing, Wang, Hua, Ma, Jing, Xu, Junfei, Sheng, Chenyi, Yang, Shuyun, Sun, Lingling, Ni, Qichao
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Breast Neoplasms - chemistry; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Cell Cycle Proteins - biosynthesis; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Line, Tumor; F-Box Proteins - biosynthesis; F-Box Proteins - genetics; F-Box Proteins - metabolism; Female; Hematology; Humans; Internal Medicine; Kaplan-Meier Estimate; Linear Models; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Paper; Pathology; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; Prognosis; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; S-Phase Kinase-Associated Proteins - biosynthesis; S-Phase Kinase-Associated Proteins - genetics; S-Phase Kinase-Associated Proteins - metabolism; Signal Transduction - genetics; Young Adult
• ISSN: 1357-0560; 1559-131X
• DOI: 10.1007/s12032-013-0735-0

S-phase kinase protein 2 (Skp2) is oncogenic and overexpressed in human breast cancer. The objective of this study was to examine the effect of early mitotic inhibitor-1 (Emi1) over-expression on Skp2 expression and related signaling pathway in breast cancer. Immunohistochemical analysis was performed in 98 human breast carcinoma samples and the data were correlated with clinicopathologic features. Furthermore, Western blot analysis was performed for Emi1 and Skp2 in breast carcinoma samples and cell lines to evaluate their protein levels and molecular interaction. We found that the expression of Emi1 was positively related with Skp2 expression ( P