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Molecular weight distribution of cellulose in primary cell walls. Investigations with regenerating protoplasts, suspension cultured cells and mesophyll of tobacco

The distribution pattern of the degree of polymerization (DP) of cellulose present in the cell walls of mesophyll- and suspension-cultured cells of tobacco was compared to that of newly synthesized 14C-labeled cellulose from regenerating tobacco protoplasts and suspension-cultured cells. The cellulo...

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Bibliographic Details
Published in:Planta 1982-06, Vol.154 (6), p.550-555
Main Authors: Blaschek, W, Koehler, H, Semler, U, Franz, G
Format: Article
Language:English
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Summary:The distribution pattern of the degree of polymerization (DP) of cellulose present in the cell walls of mesophyll- and suspension-cultured cells of tobacco was compared to that of newly synthesized 14C-labeled cellulose from regenerating tobacco protoplasts and suspension-cultured cells. The cellulose was nitrated, and, after fractionation according to differences in solubility in acetone/water, the DP pattern of labeled or unlabeled cellulose nitrate was determined by viscosity measurements. A low (DP < 500) and high DP-fraction (DP > 2500) of cellulose were predominant in the cell walls of protoplasts, suspension — cultured cells, and mesophyll cells. The average DP of the high molecular weight fraction of cellulose in the cell walls of mesophyll was higher (DP ∼4,000) than in protoplasts or suspension — cultured cells (DP 2,500—3,000). In all cell walls tested, minor amounts of cellulose molecules with a broad spectrum of a medium DP were present. Pulse — chase experiments with either protoplasts or suspension — cultured cells showed that a large proportion of the low and medium DP-cellulose are a separate class of structural components of the cellulose network. The results are discussed in relation to the organization of cellulose in the primary cell wall.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00402999