A Method of Low-Temperature Storing of Agarose Slides with Lysed Cells

A method has been developed for a long-term low-temperature storage (-10 to -15°C) of the agarose slides with nucleoids (lysed eukaryotic cells). After lysis of agarose-immobilized cells, the slides were incubated for 30 min in phosphate buffer with 50% glycerol and 100 mM EDTA, thereupon they were...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2013-10, Vol.155 (6), p.757-759
Main Authors: Kuznetsova, E. A., Dyukina, A. R., Chernigina, I. A., Sirota, N. P.
Format: Article
Language:English
Subjects:
Adult
Aged
Animals
Biomedical and Life Sciences
Biomedicine
Blood Banks
Cell Biology
Cell Extracts
Cryopreservation
DNA
DNA Damage
Female
Glycerin
Glycerol
Humans
Internal Medicine
Laboratory Medicine
Leukocytes, Mononuclear - physiology
Male
Methods
Mice
Middle Aged
Pathology
Phosphates
Sepharose - chemistry
Tissue Embedding
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method has been developed for a long-term low-temperature storage (-10 to -15°C) of the agarose slides with nucleoids (lysed eukaryotic cells). After lysis of agarose-immobilized cells, the slides were incubated for 30 min in phosphate buffer with 50% glycerol and 100 mM EDTA, thereupon they were stored in a freezer at -10 to -15°C. After long-term storage, the slides were re-incubated for 30 min in lysing solution. The measurements of the baseline and in vitro induced DNA damage in nucleoids of the human and mouse leukocytes, which had been stored in agarose slides at low temperature, showed that DNA damage level determined after a 40-day storage did not significantly differ from that of the fresh slides. The advanced storage method is simple and reliable; it opens the way to avoid cryopreservation of the biological samples and to process little by little a great number of the identically prepared slides.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-013-2245-7