Rat ceruloplasmin. Molecular cloning and gene expression in liver, choroid plexus, yolk sac, placenta, and testis

A rat ceruloplasmin cDNA clone was isolated from a rat liver cDNA library and identified by partial nucleotide sequence analysis. Rat liver ceruloplasmin mRNA levels were measured during the acute phase response to inflammation by cytoplasmic dot hybridization to ceruloplasmin cDNA. Regulation of ce...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-02, Vol.262 (6), p.2875-2878
Main Authors: Aldred, A.R., Grimes, A., Schreiber, G., Mercer, J.F.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Ceruloplasmin - genetics
Choroid Plexus - metabolism
Cloning, Molecular
DNA - analysis
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genetic engineering
Genetic technics
Liver - metabolism
Male
Methods. Procedures. Technologies
Molecular cloning
Placenta - metabolism
Rats
Rats, Inbred Strains
RNA, Messenger - metabolism
Testis - metabolism
Tissue Distribution
Yolk Sac - metabolism
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Summary:A rat ceruloplasmin cDNA clone was isolated from a rat liver cDNA library and identified by partial nucleotide sequence analysis. Rat liver ceruloplasmin mRNA levels were measured during the acute phase response to inflammation by cytoplasmic dot hybridization to ceruloplasmin cDNA. Regulation of ceruloplasmin synthesis appeared to be at the mRNA level, with the concentration of ceruloplasmin mRNA increasing significantly 12 h after induction of inflammation, reaching a maximum of 350% of normal at 36 h and returning to normal levels within 60 h. Using Northern blot analysis, extrahepatic ceruloplasmin gene expression was observed in choroid plexus, yolk sac, placenta, and testis. All these tissues are at the interface between, and possibly involved in maintaining homeostasis in, adjacent extracellular compartments. No ceruloplasmin mRNA was detected in RNA from stomach and small intestine.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)61588-1