U.v.-induced and N-methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis in Bacillus thuringiensis

The lethal and mutagenic effects of u.v. light and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on Bacillus thuringiensis were investigated. Lethality studies demonstrated that B. thuringiensis was relatively sensitive to these agents. This bacterium was mutated at the rifampicin resistance mark...

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Bibliographic Details
Published in:Mutagenesis 1987-03, Vol.2 (2), p.107-109
Main Authors: Auffray, Y., Boutibonnes, P.
Format: Article
Language:English
Subjects:
Bacillus thuringiensis
Bacillus thuringiensis - drug effects
Bacillus thuringiensis - genetics
Bacillus thuringiensis - radiation effects
Drug Resistance - genetics
Drug Resistance, Microbial - genetics
Methylnitronitrosoguanidine - pharmacology
Mutation
Rifampin - pharmacology
Ultraviolet Rays
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Summary:The lethal and mutagenic effects of u.v. light and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on Bacillus thuringiensis were investigated. Lethality studies demonstrated that B. thuringiensis was relatively sensitive to these agents. This bacterium was mutated at the rifampicin resistance marker by u.v. light and to a lesser extent by the direct acting alkylating agent MNNG. One mutant selected for its greater sensitivity to u.v. light expressed a higher frequency of mutagenesis after u.v. light treatment and appeared to be defective in an excision repair pathway. However, this mutant was only slightly mutable by MNNG in comparison with the wild-type strain. This unusual phenotype does not yet have a parallel among the radiation sensitive mutants described in other bacterial species.
ISSN:0267-8357
1464-3804
DOI:10.1093/mutage/2.2.107