Enzymatic saccharification of pretreated sunflower stalks

The sunflower stalks were pretreated by steam explosion (at 1.05 kg/ cm 2 for 0.5– 1.5 h ) and sodium hydroxide (0.25– 1.5% w/ v NaOH at 1.05 kg/ cm 2 for 0.5– 1.5 h ) using solid:liquid ratio of 0.05 g/ ml and subsequently saccharified enzymatically. Steam explosion at 1.05 kg/ cm 2 pressure for 1....

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Bibliographic Details
Published in:Biomass & bioenergy 2002-09, Vol.23 (3), p.237-243
Main Authors: Sharma, Sanjeev K, Kalra, Krishan L, Grewal, Harmeet S
Format: Article
Language:English
Subjects:
Cellulase
Enzymatic saccharification
Sodium hydroxide pretreatment
Steam explosion
Sunflower stalks
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Summary:The sunflower stalks were pretreated by steam explosion (at 1.05 kg/ cm 2 for 0.5– 1.5 h ) and sodium hydroxide (0.25– 1.5% w/ v NaOH at 1.05 kg/ cm 2 for 0.5– 1.5 h ) using solid:liquid ratio of 0.05 g/ ml and subsequently saccharified enzymatically. Steam explosion at 1.05 kg/ cm 2 pressure for 1.5 h was found to be the optimum pretreatment. Maximum enzymatic saccharification of 57.8% was observed by treating 5% ( w/ v) pretreated sunflower stalks with T. reesei Rut-C 30 cellulase ( 25 FPU/ g ) at 50°C, pH 5.0 for 72 h .
ISSN:0961-9534
1873-2909
DOI:10.1016/S0961-9534(02)00050-8