Identification of circulating porcine–human reassortant G4P[6] rotavirus from children with acute diarrhea in China by whole genome analyses

• Published in: Infection, genetics and evolution genetics and evolution, 2013-12, Vol.20, p.155-162
• Main Authors: Dong, Hui-Jin, Qian, Yuan, Huang, Teng, Zhu, Ru-Nan, Zhao, Lin-Qing, Zhang, You, Li, Rong-Cheng, Li, Yan-Ping
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antigens, Viral - genetics; Base Sequence; Child; Child diarrhea; Child, Preschool; children; China - epidemiology; diarrhea; Diarrhea - epidemiology; Diarrhea - virology; feces; Feces - virology; Full genome analysis; G4P; genes; Genome, Viral - genetics; genomics; genotype; Humans; Infant; infants; Molecular Sequence Data; Phylogeny; Porcine–human; Reassortant; Reassortant Viruses - classification; Reassortant Viruses - genetics; Reassortant Viruses - isolation & purification; Rotavirus; Rotavirus - classification; Rotavirus - genetics; Rotavirus - isolation & purification; Rotavirus A; Rotavirus Infections - epidemiology; Rotavirus Infections - genetics; Rotavirus Infections - virology; Sequence Alignment; Sequence Analysis, DNA; Swine; Swine Diseases - virology; towns; villages; Viral Proteins - genetics
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2013.08.024

•P[6] group A rotavirus (RVA) with unknown G type was found in four stool specimens.•Such P[6] RVA caused acute diarrhea in children in Guangxi Province, China.•Porcine–human reassortant G4P[6] RVA revealed by whole genomic analyses.•This G4P[6] RVA possessed 9 gene segments of porcine origin and 2 human origin.•VP6 and NSP3 might be key genes keeping reassortant circulating in human. P[6] group A rotavirus (RVA) strains identified in four stool specimens collected from children with acute diarrhea in Guangxi Province, southern China in 2010, with unknown G type were further analyzed by full genomic analysis. It was revealed by whole genome sequencing that 11 genomic cognate gene segments of these P[6] RVA strains shared almost 100% nucleotide identities and all exhibited an identical G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 genotype constellation. Phylogenetic analyses of VP7, VP1-VP4, NSP1, NSP2, NSP4 and NSP5 genes revealed that these Guangxi G4P[6] RVA strains were closely related to porcine and porcine-like human RVAs, while VP6 and NSP3 were closely related to those of common human RVAs. Interestingly, the four infants from whom these specimens were collected had come from different villages and/or towns. They had not contacted with each other and had had acute diarrhea before admitted into the same hospital. The genomic analyses and the clinical data revealed that these four Guangxi G4P[6] RVA strains from China were reassortants possessing VP6 and NSP3 gene segments of human origin yet all other nine gene segments of porcine origin. It is the first report on porcine–human reassortant G4P[6] RVA with identical genome configuration circulating in children.