Detection and quantification of mast cell, vascular endothelial growth factor, and microvessel density in human inflammatory periapical cysts and granulomas

• Published in: International endodontic journal 2012-09, Vol.45 (9), p.859-864
• Main Authors: Fonseca-Silva, T., Santos, C. C. O., Alves, L. R., Dias, L. C., Brito-Júnior, M., De Paula, A. M. B., Guimarães, A. L. S.
• Format: Article
• Language: English
• Subjects: Adult; Amyotrophic lateral sclerosis; angiogenesis; Capillaries - pathology; Cell Count; chronic periapical lesions; Coloring Agents; Connective Tissue - pathology; Cross-Sectional Studies; Dentistry; Epithelium - pathology; Female; Humans; Immunohistochemistry; Male; mast cell; Mast Cells - pathology; microvessel density; Microvessels - pathology; Periapical Granuloma - pathology; Platelet Endothelial Cell Adhesion Molecule-1 - analysis; Radicular Cyst - pathology; Tolonium Chloride; vascular endothelial growth factor; Vascular Endothelial Growth Factor A - analysis
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2012.02043.x

Fonseca‐Silva T, Santos CCO, Alves LR, Dias LC, Brito‐Júnior M, De Paula AMB, Guimarães ALS Detection and quantification of mast cell, vascular endothelial growth factor, and microvessel density in human inflammatory periapical cysts and granulomas. International Endodontic Journal, 45, 859–864, 2012. Aim  To identify and quantify mast cell (MC), vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in human periapical cysts and granulomas. Methodology  Archived samples of cysts (n = 40) and granulomas (n = 28) were sectioned and stained with toluidine blue. MCs were identified and counted. Immunohistochemical reactions were employed to evaluate the tissue expression of VEGF and vessels. MVD was estimated by determining the areas of tissue labelled with CD31 antibody. The data were analysed using the Mann–Whitney test (P