Clarification of aqueous corn extracts by tangential flow microfiltration

▶ Corn extract clarification is difficult by filtration or centrifugation. ▶ Feasible conditions were found for using tangential microfiltration. ▶ Membrane material determines fouling limitations for specific products. The effectiveness of tangential flow microfiltration for the solid/liquid clarif...

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Bibliographic Details
Published in:Journal of membrane science 2010-12, Vol.365 (1), p.123-129
Main Authors: Aspelund, Matthew T., Glatz, Charles E.
Format: Article
Language:English
Subjects:
Clarification
Membrane chemistry
Microfiltration
Protein rejection
Recombinant collagen
Tangential flow filtration
Transgenic corn
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Summary:▶ Corn extract clarification is difficult by filtration or centrifugation. ▶ Feasible conditions were found for using tangential microfiltration. ▶ Membrane material determines fouling limitations for specific products. The effectiveness of tangential flow microfiltration for the solid/liquid clarification of aqueous corn endosperm and germ extracts was examined using recombinant type I human collagen (rCollagen) and green fluorescent protein (GFP) as model proteins. We identified the effects of transmembrane pressure (TMP), crossflow rate, protein molecular weight, and membrane chemistry on permeate flux, protein rejection, and internal membrane fouling in microfiltration. In dead-end filtrations, both endosperm and germ extracts formed highly compressible cakes that provided the dominant hydraulic resistance. For tangential flow filtration using a ceramic membrane, increasing the crossflow rate had a significant beneficial effect on the permeate flux for all TMPs examined. High fouling of the ceramic membrane occurred during the filtration and this was likely due to the presence of soluble corn starch in the feed. Filtration with a ceramic membrane resulted in low rejection (
ISSN:0376-7388
1873-3123
DOI:10.1016/j.memsci.2010.08.044