MicroRNA-9 inhibits vasculogenic mimicry of glioma cell lines by suppressing Stathmin expression

The purpose of this study was to investigate the functions of microRNA-9, which is a tissue-specific microRNA in central nervous system, in the vasculogenic mimicry (VM) of glioma cell lines in vitro and in vivo.Glioma cell lines U87MG, U251 and SHG44 were transfected with microRNA-9 mimic, microRNA...

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Bibliographic Details
Published in:Journal of neuro-oncology 2013-12, Vol.115 (3), p.381-390
Main Authors: Song, Yuwen, Mu, Luyan, Han, Xuezhe, Li, Qingla, Dong, Baijing, Li, Hulun, Liu, Xiaoqian
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Blotting, Western
Brain Neoplasms - blood supply
Brain Neoplasms - genetics
Brain Neoplasms - metabolism
Cell Cycle
Cell Proliferation
Glioma - blood supply
Glioma - genetics
Glioma - metabolism
Humans
Immunoenzyme Techniques
Laboratory Investigation
Luciferases - metabolism
Male
Medicine
Medicine & Public Health
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs - genetics
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - prevention & control
Neurology
Oncology
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Small Interfering - genetics
Stathmin - antagonists & inhibitors
Stathmin - genetics
Stathmin - metabolism
Tumor Cells, Cultured
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Summary:The purpose of this study was to investigate the functions of microRNA-9, which is a tissue-specific microRNA in central nervous system, in the vasculogenic mimicry (VM) of glioma cell lines in vitro and in vivo.Glioma cell lines U87MG, U251 and SHG44 were transfected with microRNA-9 mimic, microRNA-9 inhibitor or scramble sequences. The amount of microRNA-9 and Stathmin (STMN1) mRNA was determined by quantitative real-time PCR, and the protein expression of STMN1 was determined by western blot. Cell proliferation and apoptosis were assessed. The interactions between the 3′UTR of STMN1 and miR-9 was determined by luciferase reporter assay. The VM capacity in vitro was evaluated using VM formation assay, and the rescue experiment of STMN1 was carried out in U251 cells. The in vivo experiment was applied with animal models implanted with U87MG cells.MicroRNA-9 mimic transfection reduced proliferation and increased apoptosis in glioma cell lines ( p  
ISSN:0167-594X
1573-7373
DOI:10.1007/s11060-013-1245-9