Real Time Monitoring of Fluorescent Particles in Micro-Channels by High Resolution Dual Modality Probe Imaging

Imaging of micro particles in micro-fluidic channels is one of the recent thrust areas in research as it provides more flexibility in setup and analysis compared to conventional microscopy. However, a probe based imaging scheme, with achievable high resolutions incorporating multimodal analysis is o...

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Bibliographic Details
Published in:Optics and photonics journal 2011-12, Vol.1 (4), p.197-203
Main Authors: Sathiyamoorthy, Krishnan, Mohankumar, Valiyambath Krishnan, Murukeshan, Vadakke Matham
Format: Article
Language:English
Subjects:
Channels
Emission
Flexibility
High resolution
Imaging
Microscopy
Modulation transfer function
Monitoring
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Summary:Imaging of micro particles in micro-fluidic channels is one of the recent thrust areas in research as it provides more flexibility in setup and analysis compared to conventional microscopy. However, a probe based imaging scheme, with achievable high resolutions incorporating multimodal analysis is one of the challenges researchers have been facing. In this context, this paper illustrates a simple dual modality high resolution flexible probe imaging system for imaging applications in micro/optofluidic channels. The proposed system exhibits axial and lateral resolution of about 16 mu m and 3.12 mu m respectively. This proposed system also exhibits a modulation transfer function (MTF) of about 38.42 %. The performance of the system is validated by imaging micro particles in a microchannel and obtaining the fluorescent emission spectrum simultaneously.
ISSN:2160-8881
2160-889X
DOI:10.4236/opj.2011.14031