Actin dynamics in the cortical array of plant cells

•Advanced imaging methods have been used to quantify actin turnover in live cells.•In epidermal cells, rapid assembly on filament ends is balanced with prominent severing activity.•This turnover mechanism has been termed actin stochastic dynamics.•Genetic analyses have the revealed role of several k...

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Bibliographic Details
Published in:Current opinion in plant biology 2013-12, Vol.16 (6), p.678-687
Main Authors: Henty-Ridilla, Jessica L, Li, Jiejie, Blanchoin, Laurent, Staiger, Christopher J
Format: Article
Language:English
Subjects:
actin
Actin Cytoskeleton - metabolism
Actins - metabolism
Arabidopsis
Arabidopsis - cytology
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
cells
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
hormones
Microfilament Proteins - metabolism
microfilaments
Microscopy, Fluorescence
Models, Biological
Physcomitrella
Plant Cells - metabolism
stochastic processes
Time-Lapse Imaging - methods
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Summary:•Advanced imaging methods have been used to quantify actin turnover in live cells.•In epidermal cells, rapid assembly on filament ends is balanced with prominent severing activity.•This turnover mechanism has been termed actin stochastic dynamics.•Genetic analyses have the revealed role of several key ABPs in stochastic dynamics. The actin cytoskeleton changes in organization and dynamics as cellular functions are reprogrammed following responses to diverse stimuli, hormones, and developmental cues. How this is choreographed and what molecular players are involved in actin remodeling continues to be an area of intense scrutiny. Advances in imaging modalities and fluorescent fusion protein reporters have illuminated the strikingly dynamic behavior of single actin filaments at high spatial and temporal resolutions. This led to a model for the stochastic dynamic turnover of actin filaments and predicted the actions and responsibilities of several key actin-binding proteins. Recently, aspects of this model have been tested using powerful genetic strategies in both Arabidopsis and Physcomitrella. Collectively, the latest data emphasize the importance of filament severing activities and regulation of barbed-end availability as key facets of plant actin filament turnover.
ISSN:1369-5266
1879-0356
DOI:10.1016/j.pbi.2013.10.012