Transcriptional regulation of the c-myc protooncogene by 1,25-dihydroxyvitamin D sub(3) in HL-60 promyelocytic leukemia cells

Exposure of HL-60 promyelocytic leukemia cells to calcitriol results in a decrease in steady-state levels of c-myc mRNA and induces cellular differentiation. The authors have asked whether calcitriol has a direct effect on the transcription of the C-myc gene. 1,25-Dihydroxyvitamin D sub(3) (1,25-(OH...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-01, Vol.262 (9), p.4104-4108
Main Authors: Simpson, RU, Hsu, T, Begley, DA, Mitchell, B S, Alizadeh, B N
Format: Article
Language:English
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Summary:Exposure of HL-60 promyelocytic leukemia cells to calcitriol results in a decrease in steady-state levels of c-myc mRNA and induces cellular differentiation. The authors have asked whether calcitriol has a direct effect on the transcription of the C-myc gene. 1,25-Dihydroxyvitamin D sub(3) (1,25-(OH) sub(2)D sub(3)) decreased RNA elongation in a nuclear run-off transcription assay by 4 h after treatment. The data temporally correlate inhibition of c-myc transcription with decreases in the steady-state levels of c-myc mRNA as assessed by Nortern blot analysis. The authors that the effects of 1,25-(OH) sub(2)D sub(3) on c-myc expression occurs at the transcriptional level.
ISSN:0021-9258