Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

The aim of this study was to evaluate the effects of 660 nm low-level laser therapy (LLLT) on muscle regeneration after cryolesion in rat tibialis anterior muscle. Sixty-three Wistar rats were divided into a control group, 10 J/cm(2) laser-treated group, and 50 J/cm(2) laser-treated group. Each grou...

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Bibliographic Details
Published in:Journal of rehabilitation research and development 2013-01, Vol.50 (7), p.985-996
Main Authors: Rodrigues, Natalia C, Assis, Lívia, Fernandes, Kelly R, Magri, Angela, Ribeiro, Daniel A, Brunelli, Roberta, Abreu, Daniela C C, Renno, Ana Claudia M
Format: Article
Language:English
Subjects:
Animals
Cell growth
Cold Temperature
Gene expression
Granulation Tissue - pathology
Inflammation - pathology
Injuries
Laboratory animals
Laser therapy
Lasers, Semiconductor - therapeutic use
Low-Level Light Therapy
Male
Muscle, Skeletal - injuries
Muscle, Skeletal - physiology
Muscles
Muscular system
Musculoskeletal system
MyoD Protein - analysis
Myogenin - analysis
Rats
Rats, Wistar
Regeneration
Rodents
Studies
Wound Healing - radiation effects
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Summary:The aim of this study was to evaluate the effects of 660 nm low-level laser therapy (LLLT) on muscle regeneration after cryolesion in rat tibialis anterior muscle. Sixty-three Wistar rats were divided into a control group, 10 J/cm(2) laser-treated group, and 50 J/cm(2) laser-treated group. Each group formed three subgroups (n = 7 per group), and the animals were sacrificed 7, 14, or 21 d after lesion. Histopathological findings revealed a lower inflammatory process in the laser-treated groups after 7 d. After 14 d, irradiated animals at both fluences showed higher granulation tissue, new muscle fibers, and organized muscle structure. After 21 d, full tissue repair was observed in all groups. Moreover, irradiated animals at both fluences showed smaller necrosis area in the first experimental period evaluated. MyoD immunoexpression was observed in both treated groups 7 d postinjury. Myogenin immunoexpression was detected after 7 and 14 d. The higher fluence increased the number of blood vessels after 14 and 21 d. These results suggest that LLLT, at both fluences, positively affects injured skeletal muscle in rats, accelerating the muscle-regeneration process.
ISSN:0748-7711
1938-1352
DOI:10.1682/jrrd.2012.08.0147