Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway

Objective To investigate the effect of modified Xiaochaihu Decoction (小柴胡汤, MXD) on transforming growth factor-β1/Sma- and Mad-related proteins (TGF-β1/Smads) signaling pathway in rats with chronic pancreatitis (CP) induced by dibutyltin dichloride. Methods Thirty healthy male Wistar rats were rando...

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Published in:Chinese journal of integrative medicine 2013-12, Vol.19 (12), p.935-939
Main Authors: Zhang, Shu-kun, Cui, Nai-qiang, Zhuo, Yu-zhen, Li, Di-hua, Liu, Jun-hong
Format: Article
Language:English
Subjects:
Amylases - blood
Animals
Base Sequence
Blood Glucose - metabolism
Body Weight - drug effects
Chronic Disease
Disease Progression
DNA Primers
Drugs, Chinese Herbal - therapeutic use
Lipase - blood
Male
Medicine
Medicine & Public Health
Original Article
Pancreatitis - drug therapy
Pancreatitis - metabolism
Pancreatitis - pathology
Rats
Rats, Wistar
Real-Time Polymerase Chain Reaction
RNA, Messenger - genetics
Signal Transduction - drug effects
Smad Proteins - genetics
Smad Proteins - metabolism
Transforming Growth Factor beta1 - metabolism
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Summary:Objective To investigate the effect of modified Xiaochaihu Decoction (小柴胡汤, MXD) on transforming growth factor-β1/Sma- and Mad-related proteins (TGF-β1/Smads) signaling pathway in rats with chronic pancreatitis (CP) induced by dibutyltin dichloride. Methods Thirty healthy male Wistar rats were randomly divided into the normal control group, CP group and CP+MXD-treated group. CP was induced by injection of dibutyltin dichloride (DBTC, 7 mg/kg of body weight) into the right caudal vein, and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g/kg of body weight, starting from the day after CP induction. After 28-day treatment, the n-benzoyl-tyrosyl para-aminobenzoic acid (NBT-PABA) test was carried out to evaluate exocrine pancreatic function. Then, rats were sacrificed, and pancreatic tissues were harvested for histological evaluation. In addition, the mRNA expression of TGF-β1, TGF-β1 type II receptor (TGFβRII), Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction. Results Treatment of CP with MXD improved the PABA recovery, decreased the histological lesion, and reduced the mRNA expression of TGF-β1, TGFβRII and Smad3 ( P
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-013-1656-7