Hydroxyurea-induced mitotic synchronization in Allium sativum root meristems

The synchronized divisions following a treatment with hydroxyurea (HU) — an inhibitor of DNA synthesis — were studied in root meristems of Allium sativum using two methods: autoradiography of median sections and morphological labeling with a cytokinesis inhibitor. It is shown that the second wave of...

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Bibliographic Details
Published in:Planta 1980-11, Vol.150 (1), p.26-31
Main Authors: Clain, E, Brulfert, A
Format: Article
Language:English
Subjects:
Cell cycle
Cellular metabolism
garlic
Interphase
Meristems
Mitosis
Mitotic index
Plant cells
Plant roots
Root meristems
Root tips
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Summary:The synchronized divisions following a treatment with hydroxyurea (HU) — an inhibitor of DNA synthesis — were studied in root meristems of Allium sativum using two methods: autoradiography of median sections and morphological labeling with a cytokinesis inhibitor. It is shown that the second wave of mitoses is heterogeneous: it is composed mostly of cells which have been synchronized in the S phase by the HU treatment, of cells coming from the quiescent center stimulated to enter DNA synthesis and of cells which were not blocked by the 23 h HU treatment (slow cycling cells). It is also shown that the cell cycle following the first synchronized division is considerably shortened by the synchronization procedure.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00385610