Localization and nucleotide sequence of the gene for the membrane polypeptide D2 from pea chloroplast DNA

• Published in: Plant molecular biology 1984-01, Vol.3 (4), p.191-199
• Main Authors: Rasmussen, O.F, Bookjans, G, Stummann, B.M, Henningsen, K.W
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Cell structures and functions; Chloroplast, photosynthetic membrane and photosynthesis; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; plant breeding; plant genetics
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/bf00029654

The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleotides upstream from the initiation codon and there are two sets of putative promotor sequences in the 5' flanking region. The polypeptide has a high content of hydrophobic amino acids, predominatly grouped in clusters of 20 or more residues. The 3' end of the D2 gene is overlapped by 50 nucleotides of a second open reading frame (UORF I) which is at least 369 nucleotides long. Based on current data we suggest the D2 polypeptide to be a constituent of photosystem II (PSII).