Estimation of Mutagenic Effect and Modifications of Mitosis by Silver Nanoparticles

• Published in: Bulletin of experimental biology and medicine 2013-12, Vol.156 (2), p.255-259
• Main Authors: Prokhorova, I. M., Kibrik, B. S., Pavlov, A. V., Pesnya, D. S.
• Format: Article
• Language: English
• Subjects: Antitubercular Agents - toxicity; Biomedical and Life Sciences; Biomedicine; Cell Biology; Chromosome Aberrations - chemically induced; Internal Medicine; Laboratory Medicine; Metal Nanoparticles - toxicity; Micronuclei, Chromosome-Defective - chemically induced; Micronucleus Tests; Mitosis - drug effects; Mitosis - genetics; Mitotic Index; Mutagenesis - drug effects; Mutagenicity Tests; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Nanoparticles; Onions - drug effects; Onions - genetics; Pathology; Silver - toxicity; Silver Compounds - toxicity; Tuberculosis - drug therapy; Tuberculosis - microbiology
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-013-2325-8

We analyzed mutagenic and mitosis-modifying effects of silver nanoparticles (Allium test). Chromosome aberrations and laggings and micronuclei were simultaneously registered in the same sample. Mitotic and phase indexes were calculated. No mutagenic effects were detected after treatment with silver nanoparticles in doses of 1.0, 2.5, 5.0, and 50 mg/liter. Silver nanoparticles in a concentration of 50 mg/liter significantly increased the mitotic index. Nanoparticles in a dose of 5 mg/liter induced slight, but significant increase in mitotic index, but did not affect the ratio of phase indexes. Exposure to silver nanoparticles in concentrations of 1.0 and 2.5 mg/liter was not followed by modification of mitosis.