Dopamine Disorganizes the Rhythm of Protein Synthesis Disrupting Self-Organization of Hepatocytes In Vitro

We studied dense 24-hour cultures of rat hepatocytes in serum-free medium on collagencoated slides. As before, a circahoralian rhythm of protein synthesis was observed in control cultures in a fresh medium. No rhythm was found after addition of 1-10 μM dopamine to the medium containing such cultures...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2013-11, Vol.156 (1), p.38-40
Main Authors: Brodskii, V. Y., Dubovaya, T. K., Zvezdina, N. D., Konchenko, D. S., Mal’chenko, L. A.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Biophysics and Biochemistry
Cell Biology
Cells, Cultured
Dopamine
Dopamine - pharmacology
Dopamine Agents - pharmacology
Gangliosides
Hepatocytes - drug effects
Hepatocytes - physiology
Internal Medicine
Kinetics
Laboratory Medicine
Male
Pathology
Phenols
Protein biosynthesis
Protein Biosynthesis - drug effects
Rats
Rats, Wistar
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied dense 24-hour cultures of rat hepatocytes in serum-free medium on collagencoated slides. As before, a circahoralian rhythm of protein synthesis was observed in control cultures in a fresh medium. No rhythm was found after addition of 1-10 μM dopamine to the medium containing such cultures. The rhythm was observed after addition of 0.3 μM ganglioside to pretreated-dopamine cultures. Dopamine is likely to influence the conditioning of intercellular medium with gangliosides. Deficit of this endogenous synchronizing factor in the intercellular medium blocks self-organization of the protein synthesis rhythm. Thus, in contrast to previously studied norepinephrine and serotonin, as well as gangliosides, which organized the population rhythm of protein synthesis, dopamine disorganized the rhythm, impairing direct intercellular interactions.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-013-2272-4