Differentially Delayed Root Proteome Responses to Salt Stress in Sugar Cane Varieties

• Published in: Journal of proteome research 2013-12, Vol.12 (12), p.5681-5695
• Main Authors: Pacheco, Cinthya Mirella, Pestana-Calsa, Maria Clara, Gozzo, Fabio Cesar, Mansur Custodio Nogueira, Rejane Jurema, Menossi, Marcelo, Calsa Junior, Tercilio
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Electrophoresis, Gel, Two-Dimensional; Gene Expression Profiling; Gene Expression Regulation, Plant; Metabolic Networks and Pathways; Molecular Sequence Annotation; Molecular Sequence Data; Peptide Fragments - genetics; Peptide Fragments - isolation & purification; Peptide Fragments - metabolism; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - drug effects; Plant Roots - genetics; Plant Roots - metabolism; Proteolysis; Proteome - chemistry; Proteome - genetics; Proteome - metabolism; Saccharum - drug effects; Saccharum - genetics; Saccharum - metabolism; Salinity; Salt-Tolerance - drug effects; Salt-Tolerance - genetics; Sodium Chloride - pharmacology; Stress, Physiological; Time Factors; Trypsin - chemistry
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/pr400654a

Soil salinity is a limiting factor to sugar cane crop development, although in general plants present variable mechanisms of tolerance to salinity stress. The molecular basis underlying these mechanisms can be inferred by using proteomic analysis. Thus, the objective of this work was to identify differentially expressed proteins in sugar cane plants submitted to salinity stress. For that, a greenhouse experiment was established with four sugar cane varieties and two salt conditions, 0 mM (control) and 200 mM NaCl. Physiological and proteomics analyses were performed after 2 and 72 h of stress induction by salt. Distinct physiological responses to salinity stress were observed in the varieties and linked to tolerance mechanisms. In proteomic analysis, the roots soluble protein fraction was extracted, quantified, and analyzed through bidimensional electrophoresis. Gel images analyses were done computationally, where in each contrast only one variable was considered (salinity condition or variety). Differential spots were excised, digested by trypsin, and identified via mass spectrometry. The tolerant variety RB867515 showed the highest accumulation of proteins involved in growth, development, carbohydrate and energy metabolism, reactive oxygen species metabolization, protein protection, and membrane stabilization after 2 h of stress. On the other hand, the presence of these proteins in the sensitive variety was verified only in stress treatment after 72 h. These data indicate that these stress responses pathways play a role in the tolerance to salinity in sugar cane, and their effectiveness for phenotypical tolerance depends on early stress detection and activation of the coding genes expression.