Co-culture with neurotrophic factor secreting cells induced from adipose-derived stem cells: Promotes neurogenic differentiation

•Human ADSCs have a suitable option to induce the secretion of neurotrophic factors (NTF).•NTF-secreting ADSCs release a range of growth factors whose levels in culture could promote neuronal differentiation.•The levels of secretion BDNF and NGF in NTF-secreting ADSCs was more than other neurotrophi...

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Published in:Biochemical and biophysical research communications 2013-10, Vol.440 (3), p.381-387
Main Authors: Razavi, Shahnaz, Razavi, Mohamad Reza, Kheirollahi-Kouhestani, Mahsa, Mardani, Mohammad, Mostafavi, Fatemeh Sadat
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - secretion
Adipose Tissue - cytology
Adult
Cell Separation
Coculture Techniques
Female
Human adipose-derived stem cells
Humans
Nerve Growth Factors - metabolism
Nerve Growth Factors - secretion
Neurodegenerative diseases
Neurogenesis
Neurotrophic factor secretion
Stem Cells - cytology
Stem Cells - secretion
Young Adult
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Summary:•Human ADSCs have a suitable option to induce the secretion of neurotrophic factors (NTF).•NTF-secreting ADSCs release a range of growth factors whose levels in culture could promote neuronal differentiation.•The levels of secretion BDNF and NGF in NTF-secreting ADSCs was more than other neurotrophic factors.•Co-culture with NTF-secreting ADSCs could promote neuronal differentiation more than gliogenesis. Adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) can be equally proper in the treatment of neurodegenerative diseases. However, ADSCs have practical benefits. In this study, we attempted to induce the secretion of neurotrophic factors (NTF) in human ADSCs. We then evaluated the effects of co-culture with NTF secreting cells in neural differentiation of human ADSCs. Isolated human ADSCs were induced to neurotrophic factors secreting cells. To evaluate the in vitro effects of NTF-secreting ADSCs on neurogenic differentiation of ADSCs, we used neurogenic induction medium (control group), the combination of neurogenic medium and conditioned medium, or co-cultured NTF-secreting ADSCs which were encapsulated in alginate beads (co-culture) for 7days. ELISA showed increased (by about 5 times) release of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in NTF-secreting ADSCs compared to human ADSCs. Real time RT-PCR analysis revealed that NTF-secreting ADSCs highly expressed NGF and BDNF. In addition, co-culture with NTF-secreting ADSCs could also promote neuronal differentiation relative to gliogenesis. Overall, NTF-secreting ADSCs secrete a range of growth factors whose levels in culture could promote neuronal differentiation and could support the survival and regeneration in a variety of neurodegenerative diseases.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2013.09.069