In vivo labeling of dopamine receptors: Light microscopic localization at the cellular level by means of dipping autoradiography with the agonist ( super(3)H)N-n-propylnorapomorphine

A convenient method is described for light microscopic autoradiography at the cellular level for the visualization of in vivo labeled dopamine receptors. ( super(3)H)N-n-propylnorapomorphine (( super(3)H)NPA) is administered to rats under conditions that are known to give specific and saturable accu...

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Bibliographic Details
Published in:Journal of Neural Transmission 1987-01, Vol.68 (1-2), p.15-23
Main Authors: Loopuijt, L D, Sebens, J B, Korf, J
Format: Article
Language:English
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Summary:A convenient method is described for light microscopic autoradiography at the cellular level for the visualization of in vivo labeled dopamine receptors. ( super(3)H)N-n-propylnorapomorphine (( super(3)H)NPA) is administered to rats under conditions that are known to give specific and saturable accumulation in the striatum. The autoradiograms show a high silver grain density over the striatum and a low density over adjacent external capsule and neocortex. There was a sharp delineated boundary between striatum and adjacent external capsule. The distribution of silver grains appeared to be identical with the distribution of radioactive label after similar in vivo administration of ( super(3)H)NPA in other studies.
ISSN:0300-9564