Premature preterm rupture of the membrane diagnosis in early pregnancy: PAMG-1 and IGFBP-1 detection in amniotic fluid with biochemical tests

Previable premature rupture of the membranes (pPROM), occurring before 24WG, is associated with a 25% neonatal survival rate. This terrible prognosis may lead to elective pregnancy termination on parents' request. Therefore, certain diagnosis is essential but remains difficult in about 10% of p...

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Bibliographic Details
Published in:Clinical biochemistry 2013-12, Vol.46 (18), p.1816-1819
Main Authors: Doret, Muriel, Cartier, Régine, Miribel, Juliette, Massardier, Jérome, Massoud, Mona, Bordes, Agnès, Moret, Stéphanie, Gaucherand, Pascal
Format: Article
Language:English
Subjects:
Actim PROM
Adult
Amniocentesis
Amniotic Fluid - chemistry
AmniSure
Biochemical test
Early pregnancy
Female
Fetal Membranes, Premature Rupture - diagnosis
Gestational Age
Humans
Insulin Growth Factor Binding Protein-1
Insulin-Like Growth Factor Binding Protein 1 - analysis
Limit of Detection
Placental alpha microglobulin-1
Pregnancy
Premature rupture of the membranes
Sensitivity and Specificity
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Summary:Previable premature rupture of the membranes (pPROM), occurring before 24WG, is associated with a 25% neonatal survival rate. This terrible prognosis may lead to elective pregnancy termination on parents' request. Therefore, certain diagnosis is essential but remains difficult in about 10% of patients. Bed-side biochemical tests developed to help in diagnosis had never been evaluated in early pregnancies. This study aimed to evaluate and compare the in vitro sensitivity, detection limit, reaction time and consistency of AmniSure detecting placental alpha microglobulin-1 (PAMG-1) and actim PROM detecting Insulin Growth Factor Binding Protein-1 (IGFBP-1) in amniotic fluid between 15 and 20weeks of gestation (WG). Samples of amniotic fluid were collected by amniocentesis performed between 15 and 20 completed WG in 55 patients. Dilution series were prepared and both tests were performed twice at each dilution. In vitro sensitivity, detection limit, and reaction time were evaluated and compared in serial dilution. A total of 460 AmniSure and 476 actim PROM tests were performed. Both tests' in vitro sensitivity was 100% at dilution 1:20 and remained up to 90% until dilution 1:80. In vitro sensitivities were not different at any dilution. Detection limit and consistency were similar for both tests at all dilution. Actim PROM reaction time was shorter than AmniSure at all dilutions, except 1:320 (p
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2013.10.006