In vitro translation of the double-stranded RNA genome from beet cryptic virus 1

Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K. The double-stranded RNA genome of beet cryptic virus 1 (BCV 1) has been translated, after denaturation, in a wheat germ cell-free system. Two major polypeptides, of approximate M r 52000 and 67000, were produced....

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Bibliographic Details
Published in:Journal of general virology 1987-05, Vol.68 (5), p.1417-1422
Main Authors: Accotto, G.P, Brisco, M.J, Hull, R
Format: Article
Language:English
Subjects:
antiserum
beet cryptic virus 1
Beta vulgaris
Biological and medical sciences
cryptic virus
Fundamental and applied biological sciences. Psychology
genome
in vitro
Microbiology
Phytopathology. Animal pests. Plant and forest protection
plant viruses
Plant viruses and viroids
polypeptides
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
RNA
Systematics. Structure, properties and multiplication. Genetics
Virology
wheat germ
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Summary:Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K. The double-stranded RNA genome of beet cryptic virus 1 (BCV 1) has been translated, after denaturation, in a wheat germ cell-free system. Two major polypeptides, of approximate M r 52000 and 67000, were produced. The smaller polypeptide, encoded by RNA 2 ( M r 1.15 x 10 6 ), was immunoprecipitated by an antiserum against virus particles, and is therefore likely to be the coat protein; the larger polypeptide, encoded by RNA 1 ( M r 1.36 x 10 6 ), is a likely candidate for the viral transcriptase. These two proteins represent most of the coding capacity of the BCV 1 genome (88% for RNA 1 and 82% for RNA 2), thus BCV 1 RNAs seem to act as monocistronic genes. Keywords: BCV, in vitro translation, dsRNA Permanent address: Istituto di Fitovirologia Applicata, CNR, Via Vigliani 104, 10135 Torino, Italy. Received 26 November 1986; accepted 27 January 1987.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-68-5-1417