Expression and assembly of functional bacterial luciferase in plants

The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal, reduced FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium-mediated or direct DNA transformatio...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-01, Vol.84 (1), p.131-135
Main Authors: Koncz, C, Olsson, O, Langridge, W.H.R, Schell, J, Szalay, A.A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Sciences: Cell Biology
Biotechnology
Carrots
cloning vectors
DAUCUS CAROTA
DNA
Electroporation
Enzymes
Fundamental and applied biological sciences. Psychology
GENE
gene expression
GENES
Genetic engineering
Genetic technics
GENETIC TRANSFORMATION
Genetic vectors
luciferase
Methods. Procedures. Technologies
Miscellaneous
NICOTIANA TABACUM
OPTICAL PROPERTIES
Plant cells
Plant tissues
PLANTAS
PLANTE
PLANTS
PLASMIDE
PLASMIDIOS
PLASMIDS
PROPIEDADES OPTICAS
PROPRIETE OPTIQUE
Protoplasts
Synthetic digonucleotides and genes. Sequencing
TRANSFORMACION GENETICA
transformation
TRANSFORMATION GENETIQUE
Vibrio harveyi
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Description
Summary:The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal, reduced FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium-mediated or direct DNA transformation. Simultaneous expression of the luxA and luxB genes was monitored by protein immunoblot analysis. Luciferase-mediated light emission provided evidence for the assembly of the two protein subunits into a functional dimeric enzyme in plant protoplasts, in transformed calli, and in leaves of transformed plants. Bacterial luciferase may provide a useful marker-gene system for the quantitative assay of coordinate gene expression in transgenic plants.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.1.131