Production of Selenium-Enriched Biomass by Enterococcus durans

Selenium (Se) is an essential micronutrient for several organisms, and there is an increased interest about adequate sources for dietary selenium supplementation. The aim of this study was to evaluate the selenium bioaccumulation capacity of an Enterococcus strain. The isolate LAB18s was identified...

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Bibliographic Details
Published in:Biological trace element research 2013-12, Vol.155 (3), p.447-454
Main Authors: Pieniz, Simone, Andreazza, Robson, Pereira, Jamile Queiroz, de Oliveira Camargo, Flávio Anastácio, Brandelli, Adriano
Format: Article
Language:English
Subjects:
Bioaccumulation
Biochemistry
Biomass
Biomedical and Life Sciences
Biotechnology
dietary nutrient sources
Dietary Supplements
DNA, Ribosomal
Enterococcus
Enterococcus - classification
Enterococcus - genetics
Enterococcus - metabolism
Enterococcus durans
intergenic DNA
internal transcribed spacers
Life Sciences
Nutrition
Oncology
Probiotics
ribosomal DNA
scanning electron microscopy
Selenium
Selenium - metabolism
Sodium
sodium selenite
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Summary:Selenium (Se) is an essential micronutrient for several organisms, and there is an increased interest about adequate sources for dietary selenium supplementation. The aim of this study was to evaluate the selenium bioaccumulation capacity of an Enterococcus strain. The isolate LAB18s was identified as Enterococcus durans by the VITEK® 2 system and analysis of both 16S rDNA gene sequence (JX503528) and the 16S-23S rDNA intergenic spacer (ITS). After 24-h incubation, E. durans LAB18s bioaccumulated elevated Se(IV) concentrations, reaching 2.60 and 176.97 mg/g in media containing initial amounts of 15 and 240 mg/l sodium selenite, respectively. The isolate grew optimally and had high selenium bioaccumulation at initial pH of 7.0 and 30 °C. Time course studies showed that E. durans LAB18s displayed the highest bioaccumulation of Se(IV) after 6 h of incubation. Analyses from scanning electron microscopy (SEM) demonstrated the presence of filaments connecting the cells of E. durans LAB18s cultivated in the presence of sodium selenite. It was demonstrated that a considerable amount of Se(IV) was absorbed by E. durans LAB18s. Therefore, this strain may represent an alternative source of organic dietary selenium.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-013-9818-1