The reaction of Lupinus angustifolius L. root meristematic cell nucleoli to lead

The effect of 2–48 h treatment of Lupinus angustifolius L. roots with lead nitrate at the concentration of 10 −4  M on the nucleoli in meristematic cells was investigated. In the lead presence the number of ring-shaped as well as segregated nucleoli increased especially after 12–48 h of treatment, w...

Full description

Saved in:
Bibliographic Details
Published in:Protoplasma 2011-04, Vol.248 (2), p.353-361
Main Authors: Balcerzak, Łucja, Glińska, Sława, Godlewski, Mirosław
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Cell Nucleolus - drug effects
Electron microscopy
Lead - pharmacology
Life Sciences
Lupinus - cytology
Lupinus - drug effects
Lupinus angustifolius
Meristem - cytology
Meristem - drug effects
Microscopy, Electron, Transmission
Nitrates - pharmacology
Original Article
Plant Roots - cytology
Plant Sciences
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of 2–48 h treatment of Lupinus angustifolius L. roots with lead nitrate at the concentration of 10 −4  M on the nucleoli in meristematic cells was investigated. In the lead presence the number of ring-shaped as well as segregated nucleoli increased especially after 12–48 h of treatment, while spindle-shaped nucleoli appeared after 24 h and 48 h. Lead presence also increased the frequency of cells with silver-stained particles in the nucleus and the number of these particles especially from the 12th hour of treatment. It was accompanied by significant decline of nucleolar area. Analysis of these cells in transmission electron microscope confirmed the presence of ring-shaped and segregated nucleoli. Moreover, electron microscopy revealed compact structure nucleoli without granular component. Additionally, one to three oval-shaped fibrillar structures attached to nucleolus or lying free in the nucleoplasm were visible. The possible mechanism of lead toxicity to the nucleolus is briefly discussed.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-010-0177-x