Sublethal toxicity of esbiothrin relationship with total antioxidant status and in vivo genotoxicity assessment in fish (Cyprinus carpio L., 1758) using the micronucleus test and comet assay

• Published in: Environmental toxicology 2013-11, Vol.28 (11), p.644-651
• Main Authors: Selvi, Mahmut, Çavaş, Tolga, Çağlan Karasu Benli, A., Koçak Memmi, Burcu, Çinkılıç, Nilüfer, Dinçel, Aylin Sepici, Vatan, Özgür, Yılmaz, Dilek, Sarıkaya, Rabia, Zorlu, Tolga, Erkoç, Figen
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Allethrin - analogs & derivatives; Allethrin - toxicity; Animal and plant ecology; Animal, plant and microbial ecology; Animals; Antioxidants - metabolism; Applied ecology; Biological and medical sciences; carp; Carps - genetics; Carps - metabolism; Comet Assay; Cyprinidae; Cyprinus carpio; DNA Damage - drug effects; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Erythrocytes - drug effects; esbiothrin; fish; Fresh water ecosystems; Freshwater; Fundamental and applied biological sciences. Psychology; genotoxicity; Insecticides - toxicity; micronucleus test; Micronucleus Tests; Synecology; total antioxidant status; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.20760

Esbiothrin, synthetic pyrethroid with quick activity against insects, is widely used against household pests and in public health. Despite widespread use, data on ecotoxicity and genotoxic effects are extremely scarce. The aim of the present study is to evaluate the genotoxic potential of esbiothrin on a model fish species Cyprinus carpio L., 1758 (Pisces: Cyprinidae, koi) using the micronucleus test and comet assay in peripheral blood erythrocytes. Effects of two sublethal exposure concentrations on plasma total antioxidant status (TAS mmol/L), and Hct values were examined. On the basis of the 96 h LC50 data from U.S. EPA ecotox database (32 μg/L) two sublethal exposure concentrations (5 and 10 μg/L) were used together with ethyl methanesulfonate (EMS) (5 mg/L) as positive control. Five fish were used for each dose/duration group (24, 48, and 72 h) under controlled laboratory conditions. The fish showed behavioral changes at the higher dose. Plasma TAS (mmol/L) levels decreased in 24 h; an increase was observed slightly for 48 and obviously for 72 h in both exposure doses. Similarly, hematocrit (Hct) values differed between exposure duration but no significant differences in mean values were found between groups of the same exposure time. The general trend was a rise after 48 h, which decreased afterwards. Our results revealed significant increases in the frequencies of micronuclei and levels of DNA strand breaks and thus demonstrated the genotoxic potential of this pesticide on fish, a nontarget organism of the aquatic ecosystem. To our knowledge this is the first study to report observable genotoxic effects of esbiothrin on fish. © 2011 Wiley Periodicals, Inc. Environ Toxicol 28:644–651, 2013.