Algivirga pacifica gen. nov., sp. nov., a Novel Agar-Degrading Marine Bacterium of the Family Flammeovirgaceae Isolated from Micronesia

• Published in: Current microbiology 2013-12, Vol.67 (6), p.742-747
• Main Authors: Kim, Jennifer Jooyoun, Kim, Ji Hyung, Kwon, Young-Kyung, Kwon, Kae Kyoung, Yang, Sung-Hyun, Jang, Jiyi, Heo, Soo-Jin, Park, Heung-Sik, Jung, Won-Kyo, Lee, Youngdeuk, Kang, Do-Hyung, Oh, Chulhong
• Format: Article
• Language: English
• Subjects: Agar - metabolism; Bacteria; Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Bacteroidetes - metabolism; Base Composition; Biodegradation; Biomedical and Life Sciences; Biotechnology; Chemical analysis; Cluster Analysis; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; fatty acids; Fatty Acids - analysis; glycolipids; Hydrogen-Ion Concentration; Life Sciences; Microbiology; Micronesia; Microscopy, Electron, Scanning; Molecular Sequence Data; nucleotide sequences; Phospholipids - analysis; Phylogenetics; Phylogeny; Quinones - analysis; ribosomal RNA; RNA, Ribosomal, 16S - genetics; salinity; Seawater; Seawater - microbiology; Sequence Analysis, DNA; Sodium chloride; Sodium Chloride - metabolism; Temperature; Water analysis
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-013-0429-z

An aerobic, Gram-negative, coccoid to short rod-shaped and non-flagellated marine bacterial strain S354ᵀ was isolated from seawater of Micronesia. The strain was capable to degrade agar-forming slight depression into agar plate. Growth occurred at a temperature range of 12–44 °C, a pH range of 5–9, and a salinity range of 1–7 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences suggested that S354ᵀ belongs to the family Flammeovirgaceae. The novel strain was most closely related to Limibacter armeniacum YM 11-185ᵀ with similarity of 92.5 %. The DNA G+C content was 43.8 mol%. The major fatty acids (>10 %) were iso-C₁₅:₀ and C₁₆:₁ ω5c. The predominant isoprenoid quinone was determined to be MK-7. Polar lipid profile of S354ᵀ consisted of phosphatidylethanolamine, unknown polar lipid, and unknown glycolipids. Based on the phenotypic, phylogenetic, biochemical, and physiological tests conducted in this study, S354ᵀ is proposed to represent a type strain of a novel genus and species. The 16S rRNA gene sequence of S354ᵀ is registered in GenBank under the accession number JQ639084. The type of strain Algivirga pacifica gen. nov., sp. nov. is S354ᵀ (=KCCM 90107ᵀ=JCM 18326ᵀ).