Genetic characterization and authentication of Lonicera japonica Thunb. by using improved RAPD analysis

In traditional medicine, Lonicera japonica (Thunb.) has a notable place, and it has been used for thousands of years in China, Japan, Korea and other East-Asian countries for treating cancer, inflammation, hepatic complications, influenza and wounds. However, the molecular or genetic characteristic...

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Bibliographic Details
Published in:Molecular biology reports 2013-10, Vol.40 (10), p.5993-5999
Main Authors: Fu, Junjiang, Yang, Luquan, Khan, Md. Asaduzzaman, Mei, Zhiqiang
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomarkers
Biomedical and Life Sciences
China
Cluster Analysis
Deoxyribonucleic acid
DNA
DNA, Plant - genetics
Genetic diversity
Geography
Histology
Life Sciences
Lonicera
Lonicera - genetics
Lonicera japonica
Molecular biology
Morphology
Phylogeny
Polymerase Chain Reaction
Polymorphism
Random Amplified Polymorphic DNA Technique - methods
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Summary:In traditional medicine, Lonicera japonica (Thunb.) has a notable place, and it has been used for thousands of years in China, Japan, Korea and other East-Asian countries for treating cancer, inflammation, hepatic complications, influenza and wounds. However, the molecular or genetic characteristic of this plant is not well defined. In this study, improved random amplified polymorphic DNA (RAPD) has been employed for the genetic characterization of five varieties of L. japonica collected from different geographic locations of Southern China. A total of 147 bands of DNA fragments were obtained in RAPD-PCR by using 18 primers, and the band sizes ranged from approximately 300–2,000 bp, with 3–11 amplified bands for each primer. Based on the RAPD amplification profiles, cluster dendrogram was obtained, which showed that the similarity coefficients among five varieties of L. japonica ranged from 0.59 to 0.77. To our knowledge, this is the first report of genetic characterization of L. japonica using improved RAPD analysis which has been validated by ISSR analysis, and this characterization may be useful for the preservation of genetic diversity and Lonicera population identification. Moreover, as an option, the improved method could be employed for a variety of applications in genetic diversity and fingerprinting analyses.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-013-2703-3