The E2F Transcription Factor 1 Transactives Stathmin 1 in Hepatocellular Carcinoma

Background Through data mining the Stanford Microarray Database, the stathmin 1 ( STMN1 ) transcript was found to be frequently upregulated in the hepatocellular carcinoma (HCC) with low alpha-fetoprotein level. The molecular mechanism of STMN1 upregulation in HCCs remained unclear. Methods Quantita...

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Bibliographic Details
Published in:Annals of surgical oncology 2013-11, Vol.20 (12), p.4041-4054
Main Authors: Chen, Yi-Ling, Uen, Yih-Huei, Li, Chien-Feng, Horng, Kuo-Chan, Chen, Lih-Ren, Wu, Wen-Ren, Tseng, Hong-Yu, Huang, Hsuan-Ying, Wu, Li-Ching, Shiue, Yow-Ling
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - genetics
alpha-Fetoproteins - metabolism
Blotting, Western
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Cell Cycle
Cell Proliferation
Chromatin Immunoprecipitation
E2F1 Transcription Factor - antagonists & inhibitors
E2F1 Transcription Factor - genetics
E2F1 Transcription Factor - metabolism
Female
Flow Cytometry
Follow-Up Studies
Gene Expression Regulation, Neoplastic
Humans
Immunoenzyme Techniques
Liver - metabolism
Liver - pathology
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Luciferases - metabolism
Male
Medicine
Medicine & Public Health
Mutagenesis, Site-Directed
Mutation - genetics
Neoplasm Staging
Oncology
Prognosis
Promoter Regions, Genetic - genetics
Real-Time Polymerase Chain Reaction
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Small Interfering - genetics
Stathmin - genetics
Stathmin - metabolism
Surgery
Surgical Oncology
Transcription Factor DP1 - genetics
Transcription Factor DP1 - metabolism
Transcriptional Activation
Translational Research and Biomarkers
Tumor Cells, Cultured
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Summary:Background Through data mining the Stanford Microarray Database, the stathmin 1 ( STMN1 ) transcript was found to be frequently upregulated in the hepatocellular carcinoma (HCC) with low alpha-fetoprotein level. The molecular mechanism of STMN1 upregulation in HCCs remained unclear. Methods Quantitative RT-PCR, immunoblotting, immunohistochemistry, and transfection of expression or small hairpin RNA interference plasmids, chromatin immunoprecipitation (ChIP), and quantitative ChIP assays were performed in HCC specimens or 2 distinct HCC-derived cell lines. Dual luciferase assay and site-directed mutagenesis were applied to analyze the activities of STMN1 proximal promoter region. Results STMN 1 mRNA and proteins were significantly associated with several clinicopathological features. High STMN1 or E2F1 immunoexpression was predictive of poor overall survival (OS) rate ( P  
ISSN:1068-9265
1534-4681
DOI:10.1245/s10434-012-2519-8