Phenotypic Variants of Staphylococci and Their Underlying Population Distributions Following Exposure to Stress: e77614

• Published in: PloS one 2013-10, Vol.8 (10)
• Main Authors: Onyango, Laura A, Dunstan, R Hugh, Roberts, Timothy K, Macdonald, Margaret M, Gottfries, Johan
• Format: Article
• Language: English
• Subjects: Antibiotics
• ISSN: 1932-6203
• DOI: 10.1371/journal.pone.0077614

This study investigated whether alterations in environmental conditions would induce the formation of small colony variant phenotypes (SCV) with associated changes in cell morphology and ultra-structure in S. aureus, s. epidermidis, and S. lugdunensis. Wild-type clinical isolates were exposed to low temperature (4 degree C), antibiotic stress (penicillin G and vancomycin; 0-10,000 mu g mL-1), pH stress (pH 3-9) and osmotic challenge (NaCl concentrations of 0-20%). Changes in cell diameter, cell-wall thickness, and population distribution changes (n greater than or equal to 300) were assessed via scanning and transmission electron microscopy (SEM and TEM), and compared to control populations. Our analyses found that prolonged exposure to all treatments resulted in the subsequent formation of SCV phenotypes. Observed SCVs manifested as minute colonies with reduced haemolysis and pigmentation (NaCl, pH and 4 degree C treatments), or complete lack thereof (antibiotic treatments). SEM comparison analyses revealed significantly smaller cell sizes for SCV populations except in S. aureus and S. epidermidis 10% NaCl, and S. epidermidis 4 degree C (p