Novel Species Including Mycobacterium fukienense sp. Is Found from Tuberculosis Patients in Fujian Province, China, Using Phylogenetic Analysis of Mycobacterium chelonae/abscessus Complex

• Published in: Biomedical and environmental sciences 2013-11, Vol.26 (11), p.894-901
• Main Authors: ZHANG, Yuan Yuan, LI, Yan Bing, HUANG, Ming Xiang, ZHAO, Xiu Qin, ZHANG, Li Shui, LIU, Wen En, WAN, Kang Lin
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - genetics; Base Sequence; China - epidemiology; Cluster Analysis; DNA, Bacterial - genetics; Humans; Molecular Sequence Data; Mycobacterium - classification; Mycobacterium - genetics; Mycobacterium - isolation & purification; Mycobacterium chelonae - classification; Mycobacterium chelonae - genetics; Mycobacterium chelonae - isolation & purification; Mycobacterium chelonae/abscessus complex; Mycobacterium fukienense; Mycobacterium Infections, Nontuberculous - epidemiology; Mycobacterium Infections, Nontuberculous - microbiology; Phylogeny; rRNA基因; Sequence Alignment; Species identification; Tuberculosis - epidemiology; Tuberculosis - microbiology; 中国; 分枝杆菌; 福建省; 系统进化; 肺结核; 脓肿
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2013.018

Objective To identify the novel species ‘Mycobacterium fukienense＇ sp. nov of Mycobacterium chelonoe/abscessus complex from tuberculosis patients in Fujian Province, China. Methods Five of 27 clinical Mycobucterium isolates （CIs） were previously identified as M. chelonoe/obscessus complex by sequencing the hsp65, rpoB, 165-235 rRNA internal transcribed spacer region （its）, recA and sodA house-keeping genes commonly used to describe the molecular characteristics of Mycobocterium. Clinical Mycobecterium isolates were classified according to the gene sequence using a clustering analysis program. Sequence similarity within clusters and diversity between clusters were analyzed. Results The 5 isolates were identified with distinct sequences exhibiting 99.8% homology in the hsp65 gene. However, a complete lack of homology was observed among the sequences of the rpoB, 165-235 rRNA internal tronscribed spacer region （its）, sodA, and recA genes as compared with the M. obscessus. Furthermore, no match for rpoB, sodA, and recA genes was identified among the published sequences. Conclusion The novel species, Mycobacterium fukienense, is identified from tuberculosis patients in Fujian Province, China, which does not belong to any existing subspecies of M. cheloneo/abscessus complex.